Chromatography Process Silica Gel is available in various grades, particle and pore sizes; and, are useful for all types of low-pressure, medium-pressure, and flash column chromatography. They can be applied for the clean-up and purification of a wide range of synthetic and natural compounds. It is also well known for its role in reversed-phase partition chromatography. Chromatography Process Silica Gelis a polar adsorbent and being slightly acidic in nature, it has a powerful capacity to absorb basic contents that may be present in the material that needs separation or purification. It is a technique in which the stationary phase is solid adsorbents like silica gel and activated alumina powder and the mobile phase is a liquid.